LOCUS       40924_38323        9547 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector prvFRTbgygbsd DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Ito K, Shinomura T.
  TITLE     Development and application of a new Silent reporter system to 
            quantitate the activity of enhancer elements in the type II Collagen
            Gene
  JOURNAL   Gene (2016) In press
  PUBMED    26992640
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Contact:Tamayuki Shinomura Tokyo Medical and
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan
REFERENCE   3  (bases 1 to 9547)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9547)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene (2016)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Contact:Tamayuki Shinomura Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        101..205
                     /label=AmpR promoter
     CDS             206..1063
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1237..1825
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2011..2151)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     LTR             complement(2362..2783)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     protein_bind    2850..2897
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3048..6089
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             6105..7139
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    7184..7232
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    complement(7265..7384)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7387..7782)
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(7936..8352)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    complement(8553..8752)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     LTR             complement(8815..9402)
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
ORIGIN
        1 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
       61 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      181 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      241 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      301 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      361 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      421 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
      481 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      541 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
      601 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      661 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      721 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
      781 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
      841 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
      901 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
      961 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1021 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1081 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1141 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     1981 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     2041 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     2101 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     2161 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2221 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2281 cgcgcgaggc agccagctta cctcccggtg gtgggtcggt ggtccctggg caggggtctc
     2341 ccgatcccgg acgagccccc aaatgaaaga cccccgctga cgggtagtca atcactcaga
     2401 ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt
     2461 ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt
     2521 tgtgggctct tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac
     2581 tgattggtta gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac
     2641 atctgatggt tctctagagc actgttccat ctgttcctga ccttgatctg aacttctcta
     2701 ttctcagtta tgtatttttc catgccttgc aaaatggcgt tacttaagct agcttgccaa
     2761 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
     2821 ttatcgatga gctcgcgaga tctcgactcg aagttcctat tccgaagttc ctattctcta
     2881 gaaagtatag gaacttcgga tccccagctt gggggatcga aagagcctgc taaagcaaaa
     2941 aagaagtcac catgtcgttt actttgacca acaagaacgt gattttcgtt gccggtctgg
     3001 gaggcattgg tctggacacc agcaaggagc tgctcaagcg cgatcccgtc gttttacaac
     3061 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3181 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
     3241 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
     3301 acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt
     3361 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
     3421 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
     3481 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
     3541 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg
     3601 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
     3661 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
     3721 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
     3781 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
     3841 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
     3901 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
     3961 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
     4021 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
     4081 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
     4141 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     4201 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     4261 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     4321 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     4381 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
     4441 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
     4501 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
     4561 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
     4621 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
     4681 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
     4741 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
     4801 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
     4861 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
     4921 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
     4981 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
     5041 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
     5101 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
     5161 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
     5221 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
     5281 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
     5341 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
     5401 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
     5461 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
     5521 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
     5581 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
     5641 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
     5701 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
     5761 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
     5821 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
     5881 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
     5941 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
     6001 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc
     6061 gctaccatta ccagttggtc tggtgtcagg ggatcctcgc caccatgaaa aagcctgaac
     6121 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     6181 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     6241 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     6301 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     6361 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     6421 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     6481 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     6541 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     6601 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     6661 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     6721 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     6781 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     6841 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     6901 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     6961 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     7021 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     7081 ccgatagtgg aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact
     7141 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     7201 aataaaacgc acgggtgttg ggtcgtttat tcataaacgc ggggtcgacg gtatacagac
     7261 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     7321 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     7381 caattagccc tcccacacat aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg
     7441 ctgtccatca ctgtccttca ctatggcttt gatcccagga tgcagatcga gaagcacctg
     7501 tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca tttccgatcg cgacgataca
     7561 agtcaggttg ccagctgccg cagcagcagc agtgcccagc accacgagtt ctgcacaagg
     7621 tcccccagta aaatgatata cattgacacc agtgaagatg cggccgtcgc tagagagagc
     7681 tgcgctggcg acgctgtagt cttcagagat ggggatgctg ttgattgtag ccgttgctct
     7741 ttcaatgagg gtggattctt cttgagacaa aggcttggcc atggtttagt tcctcacctt
     7801 gtcgtattat actatgcccg gtaccacccg cagagtgtac ccacaagcag taccactgct
     7861 tcaagtggta tcgctttggg gaacacgccc gggcaagctt gaggtgtggc aggcttgaga
     7921 tcagatcgga attccggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg
     7981 cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg
     8041 aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca
     8101 ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat
     8161 cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa
     8221 ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg
     8281 gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt
     8341 aacagtctgg ccctaatttt cagacaaata cagaaacaca gtcagacaga gacaacacag
     8401 aacgatgctg cagcgctgca gcagacaaga cgcgcggctt cggttccaaa ccgaaagcaa
     8461 aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa ccacatatcc
     8521 tgacggggtc ggattccaca tcgactccct tcctcaggtc gggccacaaa aacggccccc
     8581 aaagtccctg ggacgtctcc cagggttgcg gccgggtgtt cagaactcgt cagttccacc
     8641 acgggtccgc cagatacaga gctagttagc taactagtac agacgcaggc gcataacatc
     8701 aaacatagac actagacaat cggacagaca cagataagtt gctggccagc ttacctcccg
     8761 gtggtgggtc ggtggtccct gggcaggggt ctccaaatcc cggacgagcc cccaaatgaa
     8821 agacccccgt cgtgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
     8881 acgattcgga tgcaaacagc aagaggcttt attgggaata cgggtacccg ggcgacgcag
     8941 tctatcggaa gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctcggaga
     9001 gcggaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggtacag
     9061 ggtcattttc aggtccttgg ggcaccctgg aaacatctga tgattcacta gaaactgctg
     9121 agggctggac cgcatctggg gaccatctgt tcttggcccc gagccggggc aggaactgct
     9181 taccacagat atcctgtttg gcccatcact cagctgtctc atctgttctt ggccctgagc
     9241 cggggcagga accgcttacc acagatatcc tgtttggtat tcagctgttt ctttgttcct
     9301 gaccttgatc tgaacttttc tattctcagt tatgtatttt tccatgcctt gcaaagtggc
     9361 gttacttaag ctagcttgcc acctacgggt ggggtctttc aaaagcaaag aaacggaagg
     9421 gccgcggctt tggctccggt gagtgtgggg aatagggtag agtggtctaa gaggcagaag
     9481 cccgcgaatt tgggagtgtg agggggacac atttggagga cagttttcgg tgatctggta
     9541 tgaattc
//
